ToC Figure. Bacteria-nanoparticle biohybrids enhance interstitial transport of nanomedicine via self-propulsion. Herein, we show the significant effect of linkage chemistry and assembly process parameters of mixing method, duration, and volume on the density and repeatability of nanoparticle attachment. We further report the effect of nanoparticle size and attachment density on the growth rate and motility of bacteria-nanoparticle conjugates.