3.6 Impact of TV attendance on stimulated cytokine levels of polyclonally stimulated PBMCs
Stimulation with PHA and R848 led to higher levels of IL-10 in asthmatics with high daily TVA (Fig. 5A). Elevated levels were also observed for CXCL8 after stimulation with zymo. A decrease in secretion of IL-1β, IL-6, IL-12p70, IL-13, IL-17A, IL-25, IL-33, IFNα2, IFNγ and CXCL10 was observed in response to stimulation with PHA, poly I:C, R848 and zymo.
Daily long-time TVA in healthy children increased levels of IL-7, IL-12p70, IL-23A, TNF-α, IFNα2, IFNγ, CCL3, CCL5 and CXCL10 in PBMC cultures stimulated by PHA, poly I:C, R848 and zymo. A slight reduction in IL-33 and CXCL8 levels was observed for healthy subjects with longer TVA after stimulation with zymo and PHA, respectively. Cytokines that showed no significant difference between asthmatic and healthy subjects according to their daily TVA are found in Online Supplementary (S-Fig. 12 - S-Fig. 15).