Supplemental figure legends:
Figure S1: Comparison of cutoffs for epistasis for both binding affinity (left) and folding stability (right). Purple bars correspond to mutants characterized as epistatic while black indicates non-epistatic. Within the sign breakdown, blue indicates negative epistasis and red indicates positive.
Figure S2: Leave-10-percent-out test results for both binding (left) and folding (right) that demonstrate our models are robust. The top plots show the 𝚫R2 (y-axis) for the 100 runs with complexes removed (run indicated on x-axis). The color coding corresponds to a given feature. Abstracted features were combined to a single heading for that feature for simplicity. The bottom plots indicate the range of 𝚫R2 given by an error bar. The y-axis (and color coding) indicates the feature.
Figure S3: Comparison of epistasis by subcategory for categorical or boolean features. The mean value for a given subcategory is indicated by a black dot. The barplots show the histograms within the categories. In parenthesis is the number of mutation pairs belonging to each category. For the complex type, the number of complexes belonging to each category are shown in square brackets.