Statistical Analysis
Results of LFAs were evaluated in comparison to results obtained in
qPCR. For this purpose, qPCR was regarded as the standard for pathogen
detection. Accordingly, the outcome of the LFA was rated true positive
(TP), true negative (TN), false positive (FP) or false negative (FN).
Diagnostic sensitivity was calculated as TP/(TP+FN)x100. Diagnostic
specificity was calculated as TN/(TN+FP)x100.