Figure Legends
Figure 1. Dysbalanced CD4+ and CD8+ T-cell compartment in paediatric AA. A. T- and NK-cell frequency based on manually gated t-SNE plot (S1B, gates 1-8).B. CD4/CD8 T-cell ratio. C,G,H,J,K Linear regression analysis in AA children of: (C,G) blood eosinophil frequency versus (C) CD4/CD8 T-cell ratio, (G) cluster_c6 frequency with/without comorbidity, (H) CD4/CD8 T-cell ratio versus c6(TIGT+ICOS+) with intermittent symptoms or stable disease, (J) cluster_c30 frequency versus percent-predicted FEV1/FVC, (K) Fraction(Fr)-I versus Fr-II frequency, which were manually gated (I). D. tSNE-visualisation of 30 CD4+ T-cell clusters. E. Cluster_c6 and cluster_c30 frequency. F . Cluster_c6 mean marker expression.I. Dot-plot displaying CD4+ T-cell manual gating for Treg-Fr-I-III with cluster_c30 and manually gated eTregs (S1B, gate 8) overlay. A, P -value by one-way ANOVA with Benjamini-Hochberg adjustment. B,E.P -value by Mann-Whitney test.
Figure 2. Integrated T-cell signature distinguishes children with AA from HC. A. eTregs tSNE-visualization with cluster_c2 and cluster_c10. B. eTregs cluster_c2 and cluster_c10 frequency.C.Cluster_c6TIGIT+ICOS+/eTreg ratio.D. Linear regression analysis of the respective ratios versus blood eosinophil frequency in AA children. E. Principal component analysis of the AA and HC samples based on the significantly regulated features (CD8+ T-cells, CM CD8+ T-cells, CD4+ T-cell_c6, CD4+ T-cell_c30, eTreg_c2, eTreg_c10 and CD4/CD8 T-cell ratio). F. Receiver operating characteristic (ROC) curves were calculated for CD4+ T-cell cluster_c30, eTreg cluster_c2 and eTreg_c10. B,C (P value by Mann-Whitney test).