Identification of Intrinsic Proteins of Sesame OBs and Endogenous Proteases in pH 7-OB by LC-MS/MS
This was conducted according to the method by Chen, Zhang, Zhang, Kong, & Hua (2021), please check the details in this reference. Totally, 957 kinds of proteins were identified. The label-free quantification (LFQ) intensity was used to calculate the relative abundances of endogenous proteases by using equation 1 (Megger, Bracht, Meyer, & Sitek, 2013).
Relative abundance (‰) = LFQ intensity of one endogenous protease/total LFQ intensities of all identified proteins * 1000 (1)
Please note that the relative abundances were not the absolute contents of interested proteins, but they were still valuable to show the approximate contents of these interested proteins. The relative abundances of endogenous proteases were low, so they were shown as ‰. However, the relative abundances of intrinsic proteins of sesame OBs were high, so they were shown as %.