Identification of Intrinsic Proteins of Sesame OBs and
Endogenous Proteases in pH 7-OB by LC-MS/MS
This was conducted according to the method by Chen, Zhang, Zhang, Kong,
& Hua (2021), please check the details in this reference. Totally, 957
kinds of proteins were identified. The label-free quantification (LFQ)
intensity was used to calculate the relative abundances of endogenous
proteases by using equation 1 (Megger, Bracht, Meyer, & Sitek, 2013).
Relative abundance (‰) = LFQ intensity of one endogenous protease/total
LFQ intensities of all identified proteins * 1000 (1)
Please note that the relative abundances were not the absolute contents
of interested proteins, but they were still valuable to show the
approximate contents of these interested proteins. The relative
abundances of endogenous proteases were low, so they were shown as ‰.
However, the relative abundances of intrinsic proteins of sesame OBs
were high, so they were shown as %.