Demulsification of pH 7-OB into Free Oil by Sesame Endogenous Proteases
pH 7-OB was directly adjusted to pH 5, and incubated at 30−80 °C for 2 h, and centrifuged (3000g , 15 min) to float free oil. Furthermore, pH 7-OB was incubated at pH 5 and 60 °C for 0, 1, 2, 4, and 6 h, and centrifuged (3000g , 15 min) to release free oil. The free oil yield was calculated by equation 2.
Free oil yield (%) = released free oil/total lipids in pH 7-OB * 100 (2)