Supplementary material
Figure S1. Agarose gel (1%) showing F2n/R2 amplicons obtained in nested PCR for 14 leafhopper samples of study. B1: blanks, negative control; ALY, alder yellow phytoplasma; M: marker of molecular weight, 1 kb DNA ladder (RBC Bioscience). Numbers refer to the leafhopper IDs in Table S1.
Table S1. List of 227 samples of leafhoppers analyzed in 2018. All taxa belong to subfamily Deltocephalinae (Hemiptera, Cicadellidae) except the species 216 (ID) belonging to Hemiptera in family Membracidae. Each record (ID) represents a sample of 1 or more specimens of one species (columns E-F) collected in an independent collecting event (columns I-O). Results of the qPCR and nested PCR are reported in columns B-C.
Table S2. List of 16S rRNA gene sequences downloaded from GenBank and used as reference strains in this study.