Study limits
Our results are limited by several factors. This preliminary study was conducted with few individuals (1-2) for each population, and as such should be considered a baseline for future studies. The available sample sizes for every population is ten individuals. We hope to conduct future studies with all the individual samples.
Social instability was the main reason no samples of L. columnaris were collected in Nigeria. Future fieldwork in Nigeria to find and collect more populations will expand the sampling effort and allow a more robust phylogeographic study. Populations from Mt. Cameroon were under-collected because it was challenging to get the permits to work in the National Park. Finally, this study presents a partial history with the analysis of a non-recombinant marker. A more robust analysis should include nuclear genomic sequences.