Determination of repeatability of S-iELISA
The
reproducibility within and between runs of S-iELISA was evaluated, as
described
previously with some modifications (Li et
al., 2015; Lin et al., 2018).
Six control serum samples (5
IFA-positive samples and 1 IFA-negative samples) were selected for
reproducibility experiments. For intra-assay reproducibility, each serum
sample was tested in three replicates on the same plate within the same
occasion. For inter-assay reproducibility, each serum sample was tested
in three replicates on different plates of different occasions. The
results were presented as the coefficient of variation (CV), which is
the ratio of the standard deviation (SD) to the mean
OD450nm value of each group of samples (S). A CV value
criterion of 10% was used to meet the repeatability requirement of the
test.