Serum cross-reactivity of S-iELISA to other swine pathogens
To determine the cross-reactivity, porcine positive sera [All porcine positive sera were kindly provided by Professor Lang Gong (South China Agricultural University, China)] against SADS-CoV, PEDV, PDCoV, TGEV,Porcine circovirus type 2 (PCV2), Classical swine fever virus (CSFV), Pseudorabies virus (PRV), Foot-and-mouth disease virus (FMDV), Porcine reproductive and respiratory syndrome virus (PRRSV), and African swine fever virus (ASFV) were tested using the S-iELISA. Each sample was tested three times, and the mean OD450nm value was calculated to determine whether the sample was positive or negative as above described.