Serum cross-reactivity of S-iELISA to other swine
pathogens
To determine the cross-reactivity, porcine positive sera [All porcine
positive sera were kindly provided by Professor Lang Gong (South China
Agricultural University, China)] against SADS-CoV, PEDV, PDCoV, TGEV,Porcine circovirus type 2 (PCV2), Classical swine fever virus
(CSFV), Pseudorabies virus (PRV), Foot-and-mouth disease
virus (FMDV), Porcine reproductive and respiratory syndrome
virus (PRRSV), and African swine fever virus (ASFV) were tested
using the S-iELISA. Each sample was tested three times, and the mean
OD450nm value was calculated to determine whether the
sample was positive or negative as above described.