Quantification of the viral load of SARS-CoV-2
For the determination of the absolute viral load of SARS-CoV-2, a
standard ten-point curve of the SARBECO_E region (Table 1) was
generated using 1:10 dilutions from a stock of known concentration
(1x1010 to 1x101). For reverse
transcription and amplification of the target region, the SuperScript™
III Platinum® One-Step RT-qPCR System Kit (Invitrogen, cat. No.
12574035) was used in a 7500 Fast Real-Time PCR System (Applied
Biosystems®, Foster City, California, USA). The dynamic quantification
range was from 1010 to 104. The
R2 value of the generated curve was 0.997.