2,2’-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)
assay
Oils (0.5 g) were extracted with 5 ml methanol twice, and the methanol
solution was dried by gently blowing nitrogen. The residue was
resuspended in 1 ml methanol. The ABTS decolorization assay was
determined as described by Re et al. (1999). The Trolox®
(6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) standard curve
at the 5 min time point was used to determine the Trolox equivalent
(TE), and the results are reported as the µmol TE per g of extracted
oil. Analysis was done in triplicate.