Figure 5. Cytokines and macrophage changes in vitro andin vivo after NF-κb pathway blockade. (a)The cytokine levels (IL-6, IL-17A, TNF and IL-12p70) are lower in the NF-κb blockade group than in the EAE group. Anti-inflammatory cytokines, IL-4 and IL-10 are higher in NF-κb blockade group. The results in the prevention group are more significantly different. (b) Macrophages decreased in the spleen after the NF-κb blockade; Spleen M1 macrophages were higher in the EAE group than in the blockade group; Spleen M2 macrophages were higher in the EAE group than in the NF-κb blockade group. (c) In vitroproliferation and activity of M1 and M2 cells are higher in the control group than those in the NF-κb blockade group.