Cytokine cytometric bead array assay
Serum was obtained after centrifugation at 2092g for 10 min, and serum levels of IL-1β, IL-4, IL-6, IL-10, IL-12p70, IL-17A, and tumor necrosis factor(TNF) were measured using cytometric bead array(CBA) kits(BD Bioscience) according to the manufacturer’s instructions. The CBA test was repeated twice(n=6) and results were expressed in pg/ml.