Figure 2. Treated EAE with M2 macrophage that inducing in vitro and observed pathological and cytokines changes in M2-treated and control groups. (a) The proportions of macrophages and M2 macrophages were 98.48% ± 0.189% and 97.60% ± 1.407%, respectively. IL-4 and M-CSF can induce the M2 macrophages efficiently. (b) M2 macrophages were harvested on day 8 post-culture and transferred to EAE mice on days 10, 13, and 16 p.i. via the caudal vein. After adoptive transfer of M2 macrophages, the weight of the animals in the M2-treated group gradually increased after day 17 with the score decreased, while, the weight began to increase from day 22 in the control group. (c) During the peak hour, inflammatory cell infiltration and demyelination in the spinal cord are more severe in the controls than in the M2-treated group. (d) Pro-inflammatory cytokines, IL-6, IL-17A, TNF, IL-1ß and IL-12p70, are lower in the M2-treated group compared with the controls. Anti-inflammatory cytokines, IL-4 and IL-10 are higher in M2 treated group.