Histological analysis
The spinal cord was removed from the spinal canal and embedded in paraffin. Multiple horizontal sections(5-6 µm thick) of the spinal cord were stained with hematoxylin-eosin(HE) and replicate sections were stained with luxol fast blue(LFB) violet for evaluation of the extent of inflammatory cell infiltration and demyelination. The number of infiltrated inflammatory cells and the degree of demyelination in each mouse were averaged from 10 complete cross-sections of the spinal cord.