Materials
The Suppliers of the following compounds are as follows: Winfield red cigarettes (Phillip Morris, Australia); ebselen (Sapphire Bioscience, Australia); Carboxymethyl Cellulose (Sigma-Aldrich, USA); sodium pentabarbitone (Virbac, Australia); acridine orange/ethidium bromide (Invitrogen, USA); Kwik-Diff® reagent 1, fixative (Thermo Fisher Scientific, USA); Hemacolour® rapid staining of blood smear (eosin solution) (Merck, USA); Hemacolour® rapid staining of blood smear (thiazine solution) (Merck, USA); RNeasy Mini Kit (Qiagen, Germany); High Capacity RNA-to-cDNA kit (Thermo Fisher Scientific, USA); pre-developed TaqMan primers (Thermo Fisher Scientific, USA), CRP ELISA Kit (RayBiotech, USA); U46619 (Cayman Chemical, USA); acetylcholine (Thermo Fisher, USA); sodium nitroprusside (Thermo Fisher, USA); NOS3/eNOS RRID: AB_2533121 (Thermo Fisher Scientific, USA), 3-NT RRID: AB110282 (Abcam, USA); Goat anti-mouse IgG (H+L) secondary antibody, Alexa Flour Plus 488 RRID: AB_2633275 (Thermo Fisher Scientific, USA); Fluoromount-GTM, with DAPI (Thermo Fisher Scientific, USA) .