2.3 Detection of other respiratory pathogens
The common respiratory pathogens were detected with RT-PCR based on general procedure. 16 bacterial and 13 viral pathogens were detected in this study, including Mycoplasma pneumoniae (MP), Moraxella catarrhalis (MC), Chlamydophila pneumonia (CP),Streptococcus pneumonia (SP), Haemophilus influenzae (HI),Staphyloccocus aureus (SA), Pseudomonas aeruginosa (PA),Moraxella catarrhalis (MA), Klebsiella pneumoniae (KP),Legionella pneumophila (LP), Bordetella pertussis (BP),Mycobacterium avium (AV), Mycobacterium tuberculosis (TB),Acinetobacter baumannii (AB), Bordetella parapertussis(BPP), Stenotrophomonas maltophilia (SMP), Serratia marcescens (SM), influenza A (IVF-A), influenza B (IVF-B), human respiratory syncytial virus (RSV), parainfluenza (PIV, contended 1, 2, 3 and 4 four types), human metapneumovirus (HMPV), coronaviruses 229E, OC43 and NL63  (HCoV-229E, HCoV−OC43 and HCoV-NL63), human bocavirus adenovirus (HBoV), human adenovirus (HAdV), human rhinovirus (HRV), human enterovirus (HEV) and MERS-CoV, were also detected using pathogen specific one-step respiratory viruses multiplex RT-PCR detection Kit (Neuro-Hemin Biotech) and pneumobacter multiplex PCR detection Kit (Neuro-Hemin Biotech). The reliability of detection reagent was verified by a separate fluorescent real-time RT-PCR kit.