Singleplex PCR test
Singleplex PCR reactions of the 45 loci in ten species representing all major lineages of catarrhines were run on agarose gels and resulted, for all loci and species, in PCR products within the expected size range with no signs of amplifying any off-target PCR products (data not shown). Thus, locus-specificity and universal applicability of our primer set to catarrhine primates was indicated.