Flow cytometry analysis
After PEDV infection, RBCs were pre-labeled with CM-Dil Dye and stained
with a monoclonal antibody against PEDV-N protein. PBMCs were stained
with an antibody specific for porcine CD3 (BD Biosciences). To detect
PEDV, cells were resuspended in fixation/permeabilization solution (BD
Cytofix/Cytoperm kit, BD Pharmingen) and stained with antibody against
PEDV-N protein. BD FACS Calibur were used for flow cytometry and FlowJo
X.10.0.7 was used to analyze the data.