Statistical analysis
Results was performed as the mean ± SD by using SPSS 17.0 software (SPSS Inc, Chicago, IL, USA). Data was employed one-way analysis of variance (ANOVA) among multiple groups, and employed a t-tests between the two groups to determine the difference. For all analysis, p< 0.05 (*) was considered statistically significant andp < 0.01 (**) was considered highly statistically significant.