Figure legends
Figure 1. Map of the three neighbouring counties of Busia,
Bungoma, and Kakamega showing the livestock markets and slaughterhouses
from which arthropod samples were collected .
Figure 2. Melt rate profiles. (A) CCHF virus RdRp amplicons,
(B) Theileria/Babesia 18S rRNA amplicons, (C) Anaplasma16SrRNA amplicons, and (D) Rickettsia/Coxiella 16S rRNA
amplicons. PC: positive control. Ra: Rh. appendiculatus . Rd:Rh. decoloratus .
Figure 3. Maximum likelihood phylogeny of Crimean-Congo
haemorrhagic fever virus strains inferred from 34 aligned 434-nt
segments of the L-segment (RdRp gene). GenBank accession numbers and
country of origin are indicated for each sequence. Accession numbers for
sequences from this from this study are in bold. Isolation sources in
applicable sequences are also highlighted. Bootstrap values at the major
nodes are of percentage agreement among 1,000 replicates. The branch
length scale represents substitutions per site. The gaps indicated in
the branches to the Nairobi sheep disease outgroup represent 0.8
substitutions per site. The sequences from this study fall into African
genotype II as indicated by the vertical bars.
Figure 4. Maximum likelihood phylogeny of apicomplexan protozoa
inferred from 32 aligned 502-nt segments of the 18S rRNA gene. GenBank
accession numbers and isolation sources are indicated for each sequence.
Accession numbers for sequences from this study are in bold. Bootstrap
values at the major nodes are of percentage agreement among 1,000
replicates. The branch length scale represents substitutions per site.
Figure 5. Maximum likelihood phylogeny of tick-associatedCoxiella endosymbionts inferred from 33 aligned 279-nt segments
of the 16S rRNA gene. GenBank accession numbers and tick species of
origin are indicated for each sequence. Accession numbers for sequences
from this study are in bold Bootstrap values at the major nodes are of
percentage agreement among 1,000 replicates. The branch length scale
represents substitutions per site. The gaps indicated in the branches to
the L. pneumophila outgroup represent 0.12 substitutions per
site. Sequences from this study and those from GenBank fall into three
genotypes: A = Coxiella burnetii; B = Coxiella endosymbionts of Amblyomma spp. ticks; C= Coxiella endosymbionts of Rhipicephalus spp. ticks; D = Coxiella endosymbionts of Dermacentor andAmblyomma spp. ticks.