Estimation of individual-level pathogen prevalences from pooled
samples
Individual-level prevalences of pathogens detected in pooled samples
were estimated by a maximum likelihood approach in a frequentist model.
True prevalence estimates within vector populations assumed 100%
sensitivity and specificity of pooled-sample results and took into
account the number of individuals in each pool tested (Cowling et al.
1999; Williams & Moffitt, 2001). The computations were performed online
using Epitools an epidemiological calculator accessed from
https://epitools.ausvet.com.au/ppvariablepoolsize (Sergeant,
2009).