Phylogenetic analysis
All sequences were edited and aligned using Geneious alignment in
Geneious Prime version 2019.0.4 software (Biomatters, New Zealand).
Homologous sequences of reference and sequence entries closely related
with each of the individual sequences generated in this study were
identified through BLAST nucleotide searches against the GenBank nr
database (Altschup et al., 1990). Each of the datasets compiled in this
manner were aligned and the terminal regions corresponding to the primer
sequences were removed prior to phylogenetic analysis. Maximum
likelihood phylogenies were inferred for each gene using PhyML version
3.0., employing the Akaike information criterion for automatic selection
for appropriate model of evolution (Guindon et al., 2010). Trees were
visualised and edited in Figtree 1.4 (Rambaut, 2014).