2.7. Elemental composition of the exhumed nauplii.
Fine anatomical structures and elemental composition of the carcasses
were studied under SEM (Zeiss EVO-50) and associated SEM⁄EDX (energy
dispersive X-ray spectroscopy INCA Oxford 350). To prepare the samples
for SEM analysis, the nauplii were rinsed of salts and sediment
particles, as described in Naimark et al., 2018c. SEM imaging was
performed on glass supports, and SEM⁄EDX point analyses on copper
supports to allow for measurement of Al, Si, and Fe. We analyzed
separately the adhered mineral particles and the surface tissues of the
carcasses (free of bacteria and visible adhered mineral particles). This
allowed us to distinguish between the tissue intake of dissolved
elements and mere adhesion of minerals.