2.2 Capture and chemical immobilization in the Pantanal
Free ranging armadillos from Pantanal were manually captured and
anesthetized. Small species such as D. novemcinctus , E.
sexcinctus and C. unicinctus were captured by hand or using
long-handled dip nets. P. maximus were caught using cylindrical
iron traps that were placed at the entrance of burrows (Desbiez et al.,
2020). Anesthesia was induced using
a combination of butorphanol tartrate (Zoetis Indústria de Produtos
Veterinários Ltda, São Paulo/SP, Brazil; 0.1 mg/kg), detomidine
hydrochloride (Agener-União, Apucarana/PR, Brazil; 0.1 mg/kg), and
midazolam hydrochloride (Cristália, Itapira/SP, Brazil; 0.1 mg/kg), by
intramuscular injection in the hind limbs (Kluyber et al., in press).
During anesthesia, animals were identified, sexed, weighed and had their
age estimated based on Desbiez et al. (2019). Additional microchip
tagging and morphometric measurements were performed. Animals were
released in the same day at the location they were captured, immediately
after complete anesthesia recovery.