Reproductive effort
The calling effort of 391 males that survived the calling period was
measured over two consecutive days following infection cue treatment
(sample sizes for diet type and infection cue dose included in appendix
table A1). Diet had a significant effect on time spent calling (Fig. 3).
Males held on the high carbohydrate diet spent more time calling than
males maintained on the high protein diet, but the likelihood of calling
was the same for both treatments (Fig. 4B). Infection cue treatment also
influenced male calling effort (Fig. 3). We found a significant effect
of the low dose infection cue (relative to the naive controls) on both
the likelihood of calling and the amount of time spent calling (Fig. 3).
Relative to naive controls, the likelihood of calling for males injected
with the low infection cue was lower (Fig. 4A), but if they called, they
called more (Fig. 4B). None of the other infection cue treatments
differed from the naive control group with respect to either the
likelihood of calling or time spent calling.