Reproductive effort
The calling effort of 391 males that survived the calling period was measured over two consecutive days following infection cue treatment (sample sizes for diet type and infection cue dose included in appendix table A1). Diet had a significant effect on time spent calling (Fig. 3). Males held on the high carbohydrate diet spent more time calling than males maintained on the high protein diet, but the likelihood of calling was the same for both treatments (Fig. 4B). Infection cue treatment also influenced male calling effort (Fig. 3). We found a significant effect of the low dose infection cue (relative to the naive controls) on both the likelihood of calling and the amount of time spent calling (Fig. 3). Relative to naive controls, the likelihood of calling for males injected with the low infection cue was lower (Fig. 4A), but if they called, they called more (Fig. 4B). None of the other infection cue treatments differed from the naive control group with respect to either the likelihood of calling or time spent calling.