Mortality
We found considerable diet-dependent mortality during the experiment.
Specifically, there was a significant interaction between the average
amount of food eaten and diet type in their effect on mortality during
the 3-week feeding period (F1,488 = 4.8509; p =
0.0281). Mortality decreased with average mass of food eaten in both
treatments, but mortality was higher in males held on the high protein
diet, at all levels of food consumed, except at lower consumption (Fig.
2A). Additionally, both diet (F1,387 = 13.514; p< 0.001) and infection cue (F4,387 = 14.427;p < 0.001) significantly affected survival through the
2-day calling trials; in this case, the interaction term was not
significant (F4,382 = 1.032; p = 0.3905) and was
not included in the final model. Males held on the high protein diet
were the least likely to survive through the end of the 2-day calling
trial. Controlling for diet, males receiving the highest dose infection
cue also were the least likely to survive the 2-day calling trial (Fig.
2B). Males that died prior to the conclusion of the calling trials were
eliminated from further analyses of reproductive effort, immune
function, or survival.