Circulating hemocyte and microaggregation counts
Hemocytes were counted and microaggregations were observed at
400x magnification under a phase-contrast microscope in Fast-Read
102® plastic counting chambers to assess their numbers
per individual as a proxy for cellular immunity (King and Hillyer, 2013;
Stoepler et al., 2013; Duffield et al., 2018). Counting was performed
blind to diet and infection cue treatment.