Figure 2: Dynamic Sampling Platform (DSP) ESI-MS setup for direct 2D cell culture analysis. The DSP is contained in a fluidic package which allows for continuous sample treatment in a tangential flow mass exchanger orientation (right inset image) and is positioned with the ESI emitter directly in front of the MS inlet. The DSP is connected to the sampling interface, which enables direct-from-culture sampling. Localized sampling is carried out by positioning the intake capillary directly above the bottom of the cell culture, within ~50 μm of the cells, while bulk sampling is carried out by submerging the capillary just below the media surface (bottom inset image).