Virus infections and inhibitors
1 x 106 HFFF-TERTs were plated in a 25 cm2 flask. After 24 h, the medium was changed to DMEM lacking FBS but with 4 μg/ml dexamethasone, as this approach has been shown to improve infection efficiency (Tanaka et al., 1984). After 24 h, the medium was changed to DMEM containing the requisite volume of HCMV strain Merlin stock to achieve MOI 5. Cells were gently rocked (5 rpm) for 2 h, and then the medium was changed to DMEM/FBS/PS. MG132 (Sigma) at 10 μM or bortezomib (Sigma) at a range of concentrations was added to the cell culture 12 h prior to sample collection. For 12 hpi experiments, inhibitors were added to the initial viral mixture used for infection, which was replaced with drug-containing fresh DMEM after the 2 h of incubation.