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Figure 1. Genomic and phylogenetic analysis of the 56 Shiga toxin-producing Escherichia coli (STEC) strains sequenced in this study. A maximum-likelihood phylogenetic tree was generated using the core-genome Single Nucleotide Polymorphisms (SNPs) and midpoint rooted. The isolate locations are represented by color bars. The presence of a virulence gene is denoted by a pink pie. ”-” in the sequence type (ST) column indicates unrecognized ST in the MLST database; ”-” in thestx columes indicates the absence of a stx gene. The first letter in the name of each isolate indicates its animal origin: S, sheep; C, cattle.
Figure 2. Genetic relatedness of the Shiga toxin-producing Escherichia coli (STEC) isolates in this study to the human HUSEC isolates based on MLST. Each pie represents a cluster of isolates assigned to the same ST; the size of a pie is proportional to the amount of isolates in the group. The colors of and within each pie indicates the source of the isolates: green, sheep; red, cattle; blue, HUSEC. The numbers on the lines between pies indicates the number of allelic difference between two STs.