LTα1β2 induces FRC and LEC
expansion and CXCL13 production in nasal stromal cells
In addition to B cells, we found that LTβR was also expressed on
vimentin+ stromal cells in eLTs in NPs by
immunofluorescence study (Fig E5, A) and the mRNA expression levels of
LTβR was enhanced in purified stromal cells from NPs with eLTs compared
to those from NPs without eLTs and control tissues (Fig E5, B). Flow
cytometric analysis showed that LTβR expression was upregulated in four
subsets of stromal cells in NPs with eLTs (Fig E5, C and D).
We next found that LTα1β2 induced CXCL13
mRNA production in nasal stromal cells in a dose-dependent manner (Fig
5, A). LTα1β2 also induced CXCL13
protein production in nasal stromal cells (Fig 5, B). FRCs and LECs were
found to be the major stromal cell types producing CXCL13 under
LTα1β2 stimulation (Fig 5, C). Different
from the effect of IL-17A, LTα1β2induced the remodeling of nasal stromal cells towards not only FRCs but
also LECs (Fig 5, D).