Study 1. Effect of benralizumab and mepolizumab on the CRC
to histamine
Concentration-response curves (CRCs) to histamine were generated in
isolated airways. The CRCs were produced via the cumulative
administration of histamine. Airways were stimulated with histamine
until a stable level of contractility was reached for each
concentration, usually for 5-15 min. After that, the next concentration
of agonist was administered. Experiments were performed in C-, passively
sensitized airways (positive control, C+), and in passively sensitized
airways that were incubated overnight with different concentrations of
either benralizumab or mepolizumab during the sensitizing procedure.