Study 2. Effect of CRCs to benralizumab and mepolizumab on
the contractile plateau to specific histamine concentrations
Histamine was administered at concentrations inducing different levels
of contractile plateau to isolated airways that were previously
incubated overnight with different concentrations of either benralizumab
or mepolizumab during the sensitizing procedure. The concentrations of
histamine used to elicit the specific contractile plateau were the half
maximal effective concentration (EC50), the 70%
effective concentration (EC70), and the 90% effective
concentration (EC90) with respect to the maximal
contractile response [Emax] detectable in C+
isolated bronchi. The response to the different concentrations of each
specific histamine concentration was recorded until the contractile
plateau was reached, usually for 5-15 min. Experiments were performed
also in C- and C+ tissues.