Study 2. Effect of CRCs to benralizumab and mepolizumab on the contractile plateau to specific histamine concentrations
Histamine was administered at concentrations inducing different levels of contractile plateau to isolated airways that were previously incubated overnight with different concentrations of either benralizumab or mepolizumab during the sensitizing procedure. The concentrations of histamine used to elicit the specific contractile plateau were the half maximal effective concentration (EC50), the 70% effective concentration (EC70), and the 90% effective concentration (EC90) with respect to the maximal contractile response [Emax] detectable in C+ isolated bronchi. The response to the different concentrations of each specific histamine concentration was recorded until the contractile plateau was reached, usually for 5-15 min. Experiments were performed also in C- and C+ tissues.