Supplemental figure legend
Figure S1. Structural formulas and protein purification. (a) Structural formulas of 2’,4,4’,6’-tetrahydroxychalcone (THC) and naringenin. (b) SDS-PAGE of the purified GmCHS1. The calculated molecular mass of the recombinant GmCHS1 including its N -terminal tag is approximately 44 kDa. (b) The chromatogram of size-exclusion chromatography of GmCHS1. The arrowhead indicates a major fraction containing GmCHS1. The molecular mass is estimated to be approximately 80 kDa from the elution volume of the major peak, suggesting GmCHS1 exists as a homodimer in solution.
Figure S2. Representative electron densities for apo-GmCHS1 and GmCHS/nagingenin complex structures (related to figure 2). Stereo images of the 2FoFc electron density maps contoured at 1σ for the β-strands region (β7 and β8) for (a) apo-GmCHS1 and (b) GmCHS/nagingenin complex structures. Phe264 residues are labeled in both (a) and (b). The bound naringenin is shown behind the protein structure with a white stick model in (b).