Materials:
Penicillin, cefazolin, tetrazolium salt 3-(4, 5-dimethylthiazol-2-yl) 2,
5 diphenyl-tetrazolium bromide (MTT), hydrogen peroxide
(H2O2), 2’,7’-dichlorofluorescin
diacetate (DCFH-DA), Histopaque® -1077 (Ficoll), Hank’s balanced salt
solution (HBSS) and dimethyl sulfoxide (DMSO) were purchased from
Sigma-Aldrich (St, Louis, MO, USA). RPMI 1640 and trypan blue were
purchased from Invitrogen™, Life Technologies Inc. (Burlington, ON,
Canada). All other chemicals used in this study were the highest purity
commercially available.