PRX1, PRX5, and PRX6 measurements in patients with MS and NMOSD
Commercial enzyme-linked immunosorbent assay (ELISA) kits were only available for the detection of PRX1, PRX5, and PRX6 (MyBioSource, Inc, San Diego, CA, USA). CSF samples were obtained during the active disease phase (within 1 month of clinical attack and before treatment for the attack) from patients with MS (n = 16) and NMOSD (n = 16) and patients with ONDs (n = 15). Serum samples were simultaneously obtained in 10 patients with MS, 10 with NMOSD, and 10 with ONDs at the time of CSF sampling. All samples were stored at −80°C until use. The CSF and serum levels of PRX1, PRX5, and PRX6 were measured using the ELISA kits according to the manufacturer’s instructions.