PRX1, PRX5, and PRX6 measurements in patients with MS and
NMOSD
Commercial enzyme-linked immunosorbent assay (ELISA) kits were only
available for the detection of PRX1, PRX5, and PRX6 (MyBioSource, Inc,
San Diego, CA, USA). CSF samples were obtained during the active disease
phase (within 1 month of clinical attack and before treatment for the
attack) from patients with MS (n = 16) and NMOSD (n = 16) and patients
with ONDs (n = 15). Serum samples were simultaneously obtained in 10
patients with MS, 10 with NMOSD, and 10 with ONDs at the time of CSF
sampling. All samples were stored at −80°C until use. The CSF and serum
levels of PRX1, PRX5, and PRX6 were measured using the ELISA kits
according to the manufacturer’s instructions.