Symbols and Nomenclature:
ApH3.3 area of pH 3.3 iso-surface (m2)
AEX anion-exchange chromatography
CEX cation-exchange chromatography
CFD computational fluid dynamics
D diameter of impeller (m)
EOVIA the end of VI acidification
MFpH3.3 MF0.1N HCl at pH 3.3
HMW high molecular weight
HMW%,VIN the VIN pool HMW level
HMWtotal,VIN the total HMW of the VIN pool (g)
HIC hydrophobic interaction chromatography
ICH International Council for Harmonization
IgG immunoglobulin G
ILPZ integrated low-pH zone
k turbulent kinetic energy (m2s-2)
mAb monoclonal antibody
M moment (N m)
MF0.1N HCl 0.1 N HCl mass fraction
MpH3.3 HMW level (%) after a 10 min hold at pH 3.3
Np impeller power number
N agitation speed (m s-1)
PAE Protein A eluate
P /V power input per unit volume (W m-3)
T diameter of vessel (m)
SDM scale down model
SEC size exclusion chromatography
VFout1 volume fraction of outflow 1
VFout2 volume fraction of outflow 2
VI viral inactivation
VIA acidification of the viral inactivation operation
VIN neutralization of the viral inactivation operation
ρ liquid density (kg m-3)
ε turbulent dissipation rate (m2s-3)