What techniques/analyses do the researchers adopt to test their
hypothesis(es)?
The authors employ a range of different methodologies to investigate the
different structural conformations of the Q23 and Q78 HTT protein
species including cryo electron microscopy (cryoEM), small-angle X-ray
scattering (SAXS), deuterium exchange and cross-linking mass
spectrometry (MS) analyses. HTT phosphorylation motifs were mapped by MS
and then further probed via western blot analyses. Effects of
phosphorylation state and polyglutamine tract expansion are then
assessed in PRC2 activity assays and immunoprecipitation analysis.