Aim 2: Estimating the complete human sequence diversity of the homeobox transcription factor HOXC6.

Prime & go home.

Aim 3: Downstream functional characterization of constrained HOXC6 variants with a high throughput sequencing assay.

Clone library of plasmids using the essential gene protocol or technique from AB2.2 cells. Sequence that shit.

There is also the possibility of detecting disease in samples \cite{Putaporntip_2011}.